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With a pod attached, the Alto weighs about 22 grams

The proteins were separated by SDS-PAGE (Bio-Rad) in a 10% acrylamide gel, then blotted at 60 V for 2 h at 4C onto a nitrocellulose membrane

These coolants are added to refill liquids, E-cigarette liquid diluents and popular disposable E-cigarettes such as Puffbar, at levels that pose potential health risks to users, thereby requiring for these products to be prioritized for risk mitigation measures