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We should note that the smoking asthmatics mentioned in many current studies may refer to either those who started smoking after the asthma attack in childhood, or those who started smoking before the asthma attack in adulthood
Upon reaching 90% confluency, cells were rinsed with Dulbeccos phosphate-buffered saline (DPBS) without calcium and magnesium then detached using 1.5 mL of 0.25% trypsin EDTA/DPBS with polyvinylpyrrolidone for 1.5 min at 37 C
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