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1 Introduction Tobacco, a globally significant economic crop, plays a vital role in tax revenue, employment, agricultural development, and trade, particularly in developing and low-income countries ( Fertilizers, as crucial factors influencing soil ecosystems, are directly linked to physicochemical properties, enzyme activity, and microbial diversity ( A seven-year field experiment was performed in Chinas major tobacco growing regions to quantify how contrasting organic fertilizer regimes modulate soil enzyme activities, physicochemical properties, and microbial diversity, and how these changes translate into soil health, tobacco yield, and quality

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