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Nicotine, a prominent compound in tobacco, can lead to an increase in blood sugar levels by impacting insulin sensitivity and enhancing insulin resistance

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Current experiments as presented show tests for different dialysis and tag-cleavage conditions optimized at different temperatures to balance protease activity and maintain peptide stability, and comparison between additional purification strategies, such as HPLC or a second round of affinity chromatography followed by a phosphate buffer exchange, are being evaluated to maximize yield while preserving the peptides native function