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For many smokers, cigarettes are a part of their daily routinesomething they do on a coffee break, while driving, or to relieve stress

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British soldiers took up the habit during the Crimean War (18531856)

Cell culture Caco-2 epithelial colon cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and expanded in Dulbeccos modified Eagles medium (DMEM) supplemented with 20% FBS (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) to promote optimal growth and viability