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Persistent Infections: Most viruses cause acute infections, whereby the virus is rapidly produced causing symptoms over a brief period of time, but other modes of infection exist

Mobile phase A consisted of 0.01% NH4OH in water and mobile phase B of 0.01% NH4OH in methanol with a gradient starting at 5% mobile phase B which linearly increased to 90% B at 2 min and to 100% B at 2.5 min

In this study, cotinine levels decreased in the LN group compared with those in the HN group, suggesting that cotinine can serve as a biomarker of smoking with different nicotine content