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The cells were transfected with STAT3 siRNA (10 nM) using the Lipofectamine RNAiMAX (Invitrogen) following manufacturers recommendation

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As she told a gallery of reporters on Sunday after carding a three-under 67 in the final round at Lancaster Country Club, she rarely goes for alcohol (I dont need to drink because I can have a fun time without it), and she has started smoking old-fashioned cigarettes as a way to limit her nicotine consumption