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BAL is a procedure that may be performed during a bronchoscopy in which a fluid is squirted into a small portion of the lung and then aspirated to be analyzed.) Results showed that BAL samples from smokers and vapers contained high levels of all three proteases analyzed neutrophil elastase, and matrix metalloproteases 2 and 9 compared to healthy non-smokers, placing both regular smokers and e-cigarette users at risk of developing chronic lung diseases