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Rats were placed in the light compartment away from the door and then allowed to freely explore both compartments ( 2.5 Brain tissue harvesting After the final behavioral test, rats were euthanized using diethyl ether, decapitated with a guillotine and their brains were immediately removed and stored at 80C until processing

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[55] A longer tube would not allow breathing when snorkeling deeper, since it would place the lungs in deeper water where the surrounding water pressure is higher requiring significantly greater effort to inhale, and overtaxing the muscles that expand the lungs