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Western blotting The protein extracts of the lung tissues and cells were prepared using Radio Immunoprecipitation Assay (RIPA) buffer (Phygene, China) with Phenylmethanesulfonyl fluoride (PMSF) (Solarbio, China), Next, the protein concentrations were tested by a protein quantification kit (BCA Assay) (Zomanbio, China)
Symptoms can include a burning mouth, nausea, abdominal pain, salivating (drooling), diarrhoea, sweating, confusion, dizziness, agitation, increased heart rate, rapid or difficult breathing, convulsions, coma, and even death
A small clinical study investigating the pulmonary effects of vaping e-cigs containing only PG/VG found no significant difference in inflammatory cell count and cytokine expression in BALF after four weeks between e-cig users and a non-user control group 41
The White House declined to comment
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