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In order to validate the metabolic pathways and transcriptional features associated with CR production in additional data sets, we applied these transcriptional signatures to RNA-Seq data from TCGA lung adenocarcinoma, squamous NSCLC, and HCC cohorts and defined CR hi -like tumors as those belonging to the highest tertiles for the PPP and for mitochondrial urea cycle dysfunction, whereas CR lo -like tumors were defined as those belonging to the lowest tertiles for the PPP and mitochondrial urea cycle dysfunction