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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h
The program also provides education and guidance designed to help clients build coping strategies and strengthen support systems, which can matter just as much after discharge as it does during treatment
de Prestes, Nilda V
Initially believing this would have ended his magic career, Lim received surgery to repair the tendons, and he fully recovered after intense therapy
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