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All saliva samples were transferred to disposable Eppendorf tubes within 15 min of collection and frozen at 80 C until further analysis
Nicotine is a stimulant, speeding up the heart by about 20 beats per minute with every cigarette, it raises blood pressure, it is a vasoconstrictor which means it makes arteries all over the body become smaller making it harder for the heart to pump through the constricted arteries and it causes the body to release its stores of fat and cholesterol into the blood. If this isnt alarming enough just as it is, it is this process that leads to tooth loss
Pam is also the former editor of Sacramento Lifestyle Magazine
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