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(2) SMG cells: dataset integration was carried out with the top 3000 genes (although all integrated expression was calculated for all genes, given sufficient computational resources to do this) and with k.filter = 60, UMAP was created with n.neighbors = 50, min.dist = 0.3, and SNN clustering was done using the Louvain algorithm, k.param = 30, and resolution = 0.1

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Pay attention to cleaning the: If a jam occurs, you should use the cleaning probe through the spoon nozzle to remove tobacco residue in the loading chamber, and open the lid to remove remaining tobacco residue in the hopper