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Nicotine and tobacco products are addictive and can increase the risk of lung cancer, heart disease, stroke and other illnesses

Ireland was the first EU country to introduce legislation to require plain packaging for cigarettes in 2018, increasing the effectiveness of health warnings on the packets and reducing the ability of those packets to mislead consumers about smoking-related harm

The fixed samples were then quenched with 50 mM NH 4 Cl in PBS-C/M for 10 min, followed by blocking with PBS-C/M containing 0.5% BSA, 0.5% saponin, and 0.2 mg/ml sodium azide for 30 min

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