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In the review of 22nd Century Group, Inc.'s MRTP applications, the FDA evaluated data from both the company and FDA testing and found that nicotine levels in tobacco and mainstream smoke of VLN cigarettes are at least 96% lower than the majority of marketed and market-leading conventional cigarette brands

Nicotine was demonstrated to stimulate the proliferation of human colon adenocarcinoma HT-29 cells through the activation of 7-nAChR followed by the catecholamine-synthesis pathway and adrenaline release and finally, -adrenergic activation (78)

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