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Double or triple primary antibody applications were performed in Block-B buffer overnight at 4 C with dilutions as follows: KRT5 (1:500), TP63 (1:200), MKI67 (1:100), KRT8 (1:100), MUC5AC (1:500), MUC5B (1:200), KRT14 (1:200), DMBT1 (1:50), SCGB3A1 (1:20), ACTA2 (1:100), SCGB1A1 (1:500)

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it should be smooth, oily (not dry), and free of cracks or discolorations