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In agreement with the activation of systematic inflammation and immune cells, a our unpublished study demonstrated that E-cig vapor exposure over 4 months amplified CCR2 + and activated (IA + ) subsets in Ly6C high and Ly6C low macrophages, as well as CCR5 + subsets in Ly6C high macrophages in the circulation of ApoE / mice

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They exposed normal human bronchial epithelial cells to the chemicals for 24 hours