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Standards (e.g., ISO, Health Canada Intense) define puffing regimens, though real-world behavior often exceeds machine yields as smokers compensate

2014: Tobacco Control "Four hundred and sixty brands of e-cigarettes and counting: implications for product regulation": This study found that by January 2014, there were 466 brands and 7,764 unique flavors, making studying and regulating these devices a complex matter

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They lack antigen-specific receptors and are instead rapidly activated by innate cytokines that are secreted in the site of inflammation