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The fixed samples were then quenched with 50 mM NH 4 Cl in PBS-C/M for 10 min, followed by blocking with PBS-C/M containing 0.5% BSA, 0.5% saponin, and 0.2 mg/ml sodium azide for 30 min
Government authorities seized the adult ape named Shirley from a state-run zoo in Malaysia's southern Johor state last week after she and several other animals there were deemed to be living in poor conditions
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