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Materials and Methods Animals Male 3 to 4-month-old Swiss mice (3035 g) were randomly assigned into four groups ( n = 9): Ambient air (AA), HRCC, AA-plus combined exercise, and HRCC plus combined exercise

This means that in the same study, one can evaluate lung inflammation (BALF analysis, gene and protein expression analysis, histopathology), emphysematous changes (histopathology), lung function, and lung carcinogenesis (histopathology) together with carcinogen exposure (biomarkers of exposure, metabolic responses to carcinogen exposure [gene and protein expression]) and the response to DNA damage based on gene expression data

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Inflammation is closely linked to GERD as well as pre-cancerous conditions like Barretts oesophagus, where persistent acid exposure causes cellular changes in the oesophageal lining