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To confirm that PPP activity is required for CR formation, cells were treated with 6-aminonicotinamide (6-AN), a competitive inhibitor of 3-phosphoglycerate dehydrogenase (PHGDH), which catalyzes the rate-limiting step of the PPP, and the levels of CR were monitored by LC-MS/MS
These findings suggest that cigarette smoke may promote colonization and persistence of specific bacterial taxa in the human body through the biofilm formation, contributing to infections in different parts of the body
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