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All procedures and the experimental design were approved by the Ethical Committee for Animal Research of the Federal University of Ouro Preto (Protocol n 2015/35), according to the Brazilian Society of Neuroscience and Behaviour and the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals (8th edition, 2011)

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Second, ECs appear to be complementary to conventional cigarettes, meaning that they are a nicotine product in addition to conventional cigarettes, and not a substitute 47,52