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For all cell lines, both 2D and 3D, viability was assessed using CellTiter-Glo 3D (Promega), and the luminescence was measured to GloMax Explorer luminometer (Promega)

Although alterations in genetic factors have been linked with its etiology, exponential accumulation of environmental entities such as reactive oxygen species (ROS) initiate a cyclic chain reaction resulting in accumulation of cellular inclusions, dysfunctional mitochondria, and overwhelming of antioxidant machinery, thus accelerating disease pathogenesis

It is important that a child be able to follow instructions and complete assignments in a timely manner