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After 24 h, the supernatant was removed, and biofilms stained with LIVE/DEAD BacLight stain (Invitrogen) for 10 min (as performed in the Biofilm Formation assay) prior to CLSM imaging using a Leica TCS SP5 CLSM

Though not currently approved for human use, the MOTS-C peptide is becoming popular for its energy-boosting effect and is often part of peoples longevity or metabolic health stacks

Factors affecting response include: Duration of hair loss (recent loss responds better than long-standing)

The amount of weight loss will vary for each user, how quickly it works is going to vary, and other personal attributes are going to result in varying levels of success for users