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Lane 1: HEK-293 cell lysate (20 g/Lane) Lane 2: HepG2 cell lysate (20 g/Lane) Lane 3: SH-SY5Y cell lysate (20 g/Lane) Lane 4: HT-29 cell lysate (20 g/Lane) Lane 5: Human liver tissue lysate (40 g/Lane) Lane 6: Human kidney tissue lysate (40 g/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds