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For sterile filtration of peptide solutions it is advised to use a 0.22 m PES syringe filter

They were housed under controlled laboratory conditions with a 12h/12 h light/dark cycle, at a constant temperature of 25C 1C and relative humidity of 60% 10%

Despite a lack of direct evidence showing that FOXO4-DRI can eliminate senescent Leydig cells in aged mice, we observed that FOXO4-DRI treatment alleviates the testicular senescence phenotype, improves the testicular microenvironment, and increases serum testosterone levels, all of which could contribute to the therapeutic effects of FOXO4-DRI