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Under this hypoxic and high-fat dietary environment, adipose tissue exhibits significant immune cell infiltration (macrophages, T cells) accompanied by characteristic phenotypic shifts ( Local hypoxia during adipocyte hypertrophy also induces endoplasmic reticulum stress (ERS) ( 4.3.2 Cytokine-mediated ROS generation and OS Inflammatory cytokines promote ROS production through multiple pathways ( ROS activate the NLRP3 inflammasome ( 4.4 Obesity-induced gut microbiota dysbiosis and OS Through extensive studies, it has been demonstrated that obesity markedly changes the composition of microbiota ( The gut microbiota generates various reactive metabolites ( 2 O 2 ) and superoxide anions (O 2 - ), which directly modulate intestinal redox homeostasis

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Above 25 C or for 24+ hours, assume degradation and discard

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