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This is because the turnover of skin cells takes time, and the "old" pigment must naturally flake away to reveal the "new" tone beneath

Intramuscular injection near an injury site (a common self-administration approach) adds a theoretical local concentration benefit that has some support in animal wound models but has not been tested head-to-head against subcutaneous delivery in a controlled trial

And in animal experiments, what theyve done is theyve said, Okay, lets give these animals inhibitors of their mitochondria at specific locations and see what methylene blue does. And if you dont have any inhibitors, and if the animal is genetically healthy, then you add methylene blue, they get less ATP, so the mitochondria is less effective at converting food to usable energy

Acetaminophen activation by human liver cytochromes P450IIE1 and P450IA2