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Histopathological evaluation was performed using a semi-quantitative scoring system previously described in the literatre.23 Each slide was examined independently by two blinded histologists who were unaware of the experimental groups

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On the other hand, substituting sonication for BugBuster did make a difference, and a ~ 3.5-fold increase in the yield with sonication was observed (see LSB-3 + BB versus LSB-3 + SN in Fig