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On the other hand, the concept of non-genomic or cytoplasmic response was introduced in 1967, when Szego and Davis observed an increase in cyclic adenosine monophosphate (cAMP) in the MCF-7 breast cancer cell line pretreated with estrogens (Szego and Davis, 1967)

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Exercise capacity was determined by a single maximal exercise test to voluntary fatigue on a motorized treadmill (Exer 3/6, Columbus Instruments) in females [Young ( n = 10), Old Con ( n = 17), and Old mAb ( n = 22)] and males [Young ( n = 10), Old Con ( n = 9), and Old mAb ( n = 9)]