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After adding 340 L 4 C cold mixture of methanol and acetonitrile (ACN) (25:75, v/v) and 50 L of a 2 M D 9 -trimethylamine-N-oxide (Cambridge Isotopes Laboratories, Tewksbury, MA, USA) solution in methanol, the protein was precipitated by vortex-mixing for 30 s followed by 10 min incubation
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Many fibromyalgia patients take medications such as tricyclic antidepressants or SNRIs (e.g., duloxetine), some of which may contribute to weight gain
Total RNA was isolated from approximately 100 mg of brain tissue utilizing TRIzol reagent (Invitrogen, Carlsbad, CA, United States), and RNA concentration and purity were determined using a NanoDrop spectrophotometer
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