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The homogenate was centrifuged at 22000 g for 15 min at 4 C, and the supernatant was collected for analyses of ascorbate and glutathione

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6e, all three GSSG-enhancing chemicals increased the levels of IKK--SSG adducts, suggesting that GSSG accumulation restrained IKK- activity by promoting IKK- S -glutathionylation, leading to subsequent NF-B suppression