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Reduced calorie intake can lead to: Loss of lean muscle mass Low protein intake Vitamin deficiencies (especially B vitamins and Vitamin D) For patients using GLP-1s for weight loss, maintaining proper nutrition is essential for healthy, sustainable results

The mixture was added to the dish containing cells in 1 ml of media and incubated for 24 h at 37 C prior to harvesting for nuclear preparation and Electrophoretic Mobility Shift Assay

A total of 250 ng of target DNA was used per reaction

These complex clinical manifestations and complications necessitate a thorough exploration of PCOS pathogenesis and its potential therapeutic targets, marking it as a key topic in current biomedical research