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However, activation of caspase-3 was not essential for the execution of apoptosis-like cell death of RBCs, resulting in the hypothesis that caspases were required for appropriate erythropoiesis [23]

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Determination of the nuclear maturation stage After maturation, the oocytes were washed in PBS-PVA, denuded from cumulus cells by gentle pipetting, fixed in 0.5% glutaraldehyde (v/v) for 15 min and stored at 4 C

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