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Suitable for studies involving peptide characterization and method development in model systems
Qualitative assessment of nitric oxide production by fluorescent dye 3-Amino-4-aminomethyl-2,7-difluorescein, diacetate (DAF-FM DA, Sigma) HUVECs grown in M199 based culture medium was stimulated with 1.0 g/ml BPC 157 for 30 min to induce the nitric oxide generation
It changes fast, its easy to measure, and it responds quickly to lifestyle shifts
The simplest way to do this is to use a standard concentration, like the 1mg/ml ratio we discussed earlier
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