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Such coordinated signaling ensures rapid Ca 2+ uptake into the mitochondrial matrix, activation of TCA cycle enzymes, and stimulation of ATP synthesis ( 2+ fluctuations, promotes controlled secretion, and protects the cell from the spread of pathological calcium signals ( 2+ imaging, NADH autofluorescence, ATP sensors) rather than direct dynamic visualization of individual MAM microdomains, and thus cannot fully resolve the causality between structural contacts and functional responses

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This is a separate and still investigational line of research rather than an established use, and it should not be confused with the fat-loss mechanism that the bulk of the clinical work has focused on