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Subsequently, the reaction system was diluted with ethyl acetate (200 mL 3), followed by washing with water (100 mL 3) and saturated sodium chloride solution (100 mL 3)

Similarly, in humans, NAD + levels decrease with aging and intake of unbalanced nutrients, such as fat and protein-rich diets, in organs such as the skin [49], skeletal muscle [50], liver [45], and brain [51,52] and in the plasma [53,54] and monocytes [55]

RNAi of cye-1 and chk-1 genes also showed the Glp phenotype (14% and 24%, respectively) in eri-1 mutants (Fig

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