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The substrates were then coated for 2 h at 37 C with 30 g/ml of mouse collagen IV (Cultrex, #3410-010-02, Bio-Techne, Germany), 30 g/ml of type I collagen from calf skin (Collagen G, L7213, Sigma-Aldrich, USA), or 10 g/ml of bovine plasma fibronectin (F1141, Sigma-Aldrich, USA) diluted in sterile D-PBS (Gibco, USA)

Dogs with true carnitine-responsive cardiomyopathy often show measurable improvement in cardiac function over several months of supplementation

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