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serum was isolated by centrifugation (10,000 rpm, 10 min, 4 C) and analyzed for alanine aminotransferase (ALT) and aspartate aminotransaminase (AST) by the University of Pittsburgh Medical Clinical Laboratory

At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

Mice received intraperitoneal injections of this solution at a dose of 3 mg/kg body weight

fimbriata spores to examine the resistance