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For CRISPRa to work, single-guide RNAs (sgRNA) are designed to target regions between 1 to 1000 bp upstream of the transcription start site (TSS) within the promoter region of the target gene

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Taken together, these studies suggest that C-terminal truncated Syn variants may act as molecular decoy sequestering heterogeneous molecules, both proteins and membrane organelles, in their vicinity through nonfunctional interaction, leading to diffuse cellular aggregates or LB nucleation, as observed in various neurodegenerative disorders 83