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In vitro transcription with 3-Cy3 labelling Cold in vitro transcription was performed in a volume of 50 l containing 1 g DNA template (annealed oligonucleotides), 1 mM NTPs solution, 100 U RiboLock RNase Inhibitor (Thermo Fisher Scientific), 1x Transcription Buffer and 500 U T7 RNA Polymerase (NEB)

While several biomarkers may represent strong potential candidates as early indicators of liver injury, the existing literature is limited by studies that have relatively small sample numbers and relatively sparse sampling strategies

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Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelialmesenchymal transition in drug-resistant lung cancer