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DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted

Bacteriostatic Water provides a suitable multi-use reconstitution vehicle for PEG MGF and the PEGylation of the MGF E-domain peptide provides aqueous solubility compatible with direct Bacteriostatic Water reconstitution without the acetic acid acidification sometimes required for unconjugated peptides

Store GHK-Cu after reconstitution in conditions that preserve the copper-peptide bond, and you preserve the biological activity that makes the compound worth using in the first place