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For preparation of the Mn/TI@mHSA-N control, a similar procedure was followed, substituting NHS-C7-NHS for NHS-TK-NHS

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All subjects attended a study day at baseline, 9 weeks (representing the end of the weight loss phase) and 18 weeks (end of weight maintenance phase) for a meal tolerance test (MTT) (Ensure Plus (220 ml), Total energy: 1380 kJ, 44.4 g carbohydrate, 10.8 g fat, 13.8 g protein)

I say, start with electrolytes