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The ATP-dependent pathways require anserine synthetase, as well as carnosine synthetase plus carnosine 1-methyltransferase (Wu 2018)

GPx1 deficiency may also (partially) protect liver hepatocytes against acetaminophen-mediated toxicity by mechanisms that decrease protein nitration and cell death, whereas GPx1 overexpressing mice are more sensitive to acetaminophen-mediated hepatotoxicity, in part due to an exhaustion of GSH [175]

The compound shows effectiveness across a wide dose range in animal studies (10 ng/kg to 10 mcg/kg producing comparable results), suggesting that more is not necessarily better

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