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Repetitive behavior was also assessed by self-grooming testing, which was performed by habituating the test mouse to a beaker followed by observation of the time spent grooming in the beaker, with increased grooming time indicative of more repetitive behavior

The goal is an improved metabolic profile: reduced risk or reversal of type 2 diabetes, reduction in fatty liver disease, reduction in cardiovascular disease, and reduced risk of other co-morbidities of obesity including certain cancers

This assay was applied to both cultured cells and frozen tissue sections, with tissue sections counterstained with Nuclear Fast Red for better visualization

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