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Twenty-four hours later, media was exchanged with 3 mL of DMEM +dFBS (or RPMI +pyruvate +dFBS / McCoys 5A +dFBS ) and 300 ng/mL of Dox (no Dox was added to 786-O lenti cell lines which express LUC or 87 Cr GPDH 705 constitutively), and cells were then returned to the incubator

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We demonstrated that Gln promotes proliferation by maintaining GSH-ROS balance, and refined the Gln/c-Myc/miR-29b-3p/GGCT regulatory axis, which links Gln availability to GGCT expression