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DNA fragmentation percentage in each sample was expressed by the formula: Quantitative real-time polymerase chain reaction of mitochondrial transcription factor A (mtTFA), tumor necrosis factor-alpha (TNF-), and insulin-like growth factor-1 (IGF-1) genes RNA was extracted from heart and lung tissues using the QIAmp miRNAsy mini kit (QIAGEN, Hilden, Germany) as directed by the manufacturer
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