Immunoblotting was performed with the standard method using the following antibodies: anti-SARS-CoV-2 Spike (1A9, GTX632604, mouse monoclonal antibody, GeneTex), anti-SARS-CoV-2 Spike S2 (40590-T62, rabbit polyclonal antibody, Sino Biological), Cleaved SARS-CoV-2 Spike (Ser686) (84534, rabbit polyclonal antibody, Cell Signaling Technology), anti-GAPDH (3H12, M1713, mouse monoclonal antibody, Medical & Biological Laboratories), goat anti-mouse IgG antibody conjugated with horseradish peroxidase (Cat# SA00001-1, Proteintech Group), donkey anti-rabbit IgG antibody conjugated with horseradish peroxidase (Cat# NA934, Cytiva)
Incorporating these supplements into your routine can enhance the benefits of intermittent fasting and support your weight management goals
It's a long-term marker of omega-3 status
doi: 10.3389/fimmu.2015.00508