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The effects of iron deficiency in tissues and reduced activity of iron-containing enzymes, such as growth retardation, abnormal behavior, and physical deficiency, can therefore be gradually corrected after active iron supplementation in iron-deficient animals, in addition to accelerating hemoglobin synthesis

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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water